Pluripotent stem cells (PSC) are a promising cell source for creating therapeutics because these cells can be expanded indefinitely and differentiated into all cell types in the body. Industrializing these therapies will require the development of scalable and controlled suspension bioreactors for PSC-based cell therapy manufacturing.

Cell aggregation refers to the process in tissue formation where cells of the same type bind together to form a cluster, enabling the tissue to fulfill its intended function. This process …

During the aggregation process in vitro, the mitosis of MSCs is gradually suppressed with the convergence and cell-cell contact of MSCs. [ 24, 57, 67 ] Researchers have constructed engineered MSC aggregates which partially mimic or reconstitute the key features of mesenchymal aggregation in vitro.

Protein aggregation is a multi-step process that involves various aggregation species with different conformational states. It is important to distinguish insoluble aggregates from …

MOAG binds to negatively charged residue clusters distal from APRs on aggregation-prone proteins and expedites aggregation into inclusions, thereby limiting toxicity of the aggregation process to ...

The process of cell aggregate formation, typically via reaggregation of singularized cells, is a critical initial step for the generation of many organoids.

Cell aggregation refers to the process in tissue formation where cells of the same type bind together to form a cluster, enabling the tissue to fulfill its intended function. This process involves cell-to-cell recognition and adhesion, with aggregation factors …

Cell migration plays a central role in a wide variety of biological phenomena. In embryogenesis, cellular migrations are a recurring theme in important morphogenic processes ranging from gastrulation to development of the nervous system. Migration remains prominent in the adult organism, in normal physiology as well as pathology. In the inflammatory response, …

One of the most fundamental aspects of developmental biology is the ability of cells to aggregate and form tissue patterns. Based on a lattice model, we investigate the underlying dynamics of the aggregation process incorporating three different kinds of cellular interactions, namely, due to cell-cell adhesion mediated by physical contacts, mechanical …

Whereas inhibition of cadherin function prevents cell aggregation and islet formation, loss of N-CAM only impairs the cell sorting process, so that disorganized islets form. Similarly, whereas mutant mice that lack N-cadherin die early in development, mutant mice that lack N-CAM develop relatively normally, although they do have some defects in ...

This indicated that the addition of the polymer effectively prevented fibrillation, demonstrating its complete blocking effect on the aggregation process induced by thermal stress, thus underscoring the significant potential of PSPB-based polymers to protect insulin from aggregation under thermal conditions.

Protein aggregation is a feature of numerous neurodegenerative diseases. However, regulated, often reversible, formation of protein aggregates, also known as condensates, helps control a wide range of cellular activities including stress response, gene expression, memory, cell development and differentiation.

We show how three leading pathways of cell-cell interactions due to direct cell adhesion contacts, matrix-mediated mechanical interactions, and chemical signaling affect the aggregation dynamics.

The cell-to-cell and cell-to-medium interactions influence the aggregation process and the design of the kernel (K(cdot )). This falls outside the scope of this paper but is a subject for ...

Cell aggregation is mainly the result of differential adhesion hypothesis (DAH), interfacial tension, and cytoskeletal contraction. Self-assembly and self-organization of the cells is predetermined by the type of cells and their preculture time.

The aggregation of red blood cells (RBCs) is a complex phenomenon that strongly impacts blood flow and tissue perfusion. Despite extensive research for more than 50 years, physical mechanisms that ...

Protein aggregation is a highly complex process in itself so it should be kept in mind that a single technique cannot be enough for the complete characterization of the chain of events taking place in the process of aggregation and thus the …

Live cell monitoring system (LiMSy) In this study, we developed a system able to continuously monitor the single cell aggregation process in an automated and high-throughput manner.An automated stage scanning microscope, consisting of a bright-field objective (Olympus IX53) with 4X magnification and a SC30 Colour Camera (Olympus), was used for image …

Drugs that influence the aggregation process may therefore interfere with cell–cell adhesion as well as with cell growth or de-adhesion. In this way, the slow aggregation assay on agar may also become a useful tool for …

120 rowsCell aggregates are important tools in the study of tissue development, permitting correlation of cell–cell interactions with cell differentiation, viability, and migration, as well as …

Red blood cell (RBC) aggregate to form two- and three-dimensional structures when suspended in aqueous solutions containing large plasma proteins or polymers; this aggregation is reversible and shear dependent (i.e., dispersed at high shear and reformed at low or stasis). The extent of aggregation is the main determinant of low shear blood ...

1 INTRODUCTION. Cell–cell aggregation, which biologically appears in cell recognition and cell communications, has been widely known as an essential process of cell–cell interactions, formation of multicellular organisms, and integration of biofunctions.

More recently, seeded α-syn PFFs induced the aggregation of endogenous α-syn while significantly increasing the secretion of nanoscopic α-syn aggregates by disrupting the protein homeostasis of SH-SY5Y cells and in Cascella et al. (2021), where they used rat primary hippocampal cells and SH-SY5Y cells (as well as hiPSC-derived DA neurons ...

While heparin has been shown to eliminate cell aggregation in suspension adaptations of insect and HEK293 cells for virus-based cell cultures, the role of heparin in long period serum-free suspension adaptation of the anchorage-dependent Chinese hamster ovary (CHO) cell lines remains inconclusive. In this paper, we explore the potential application of …

S. aureus cells aggregate when pH is decreased and salt or protein concentrations are increased. (a) Aggregation of S. aureus cells as a function of pH in water with 0.015% and 0.5% of NaCl and in ...

Cell aggregation within the pancreas and liver (adapted from ): (a) islet of Langerhans; (b) liver (stained for glycogen deposits); (c) islet of Langerhans (high magnification, stained to differentiate different endocrine cell types). Open in new tab Download slide.

Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell ...

Cell aggregation is a dynamic and complex process ubiquitous in life. It occurs in morphogenesis, from organisms situated at the border between single and multicellular life forms, for instance Dyctyostelium discoideum [1], to mammals in which tissues are formed by the assembly of migratory and similar cells into cohesive groups [2].Also, aggregation disorders …

Cell aggregation refers to the process of cells coming together and forming clusters, which can be observed in various biological contexts such as protein aggregation associated with diseases like neurodegenerative disorders and diabetes. AI generated definition based on: ...

In the process of division, plant cells cultivated in vitro in liquid nutrient media do not pass into the culture medium in the form of single cells, but they tend to maintain connections with each other, forming groups of cells of different sizes. ... Cell aggregation is affected by the presence of growth regulators in the medium. High levels ...